Research Article
Comparison of the Loop-Mediated Isothermal Amplification (LAMP) and the Kato-Katz Techniques in the Diagnosis of Schistosoma mansoni in Burkina Faso
Issue:
Volume 9, Issue 2, June 2024
Pages:
30-36
Received:
1 May 2024
Accepted:
21 May 2024
Published:
3 June 2024
Abstract: Intestinal schistosomiasis or intestinal bilharzia, mainly caused by Schistosoma mansoni, is one of the most common parasitic diseases in the world, and a neglected tropical disease (NTD). It ranks first among water-borne diseases and is the 2nd most endemic parasitic disease after malaria and according to the World Health Organization (WHO), schistosomiasis is transmitted in more than 78 countries and territories in tropical and subtropical regions, and more than 250 million people are infected, mainly in Africa. Kato Katz (KK) remains the standard technique for diagnosing this disease. A promising new approach, loop-mediated isothermal amplification (LAMP), may be needed in developing countries such as Burkina Faso. Thus, the aim of this study was to compare the LAMP technique and the Kato-Katz technique in the diagnosis of Schistosoma mansoni in Burkina Faso. 52 stool samples were collected from patients in the town of Bobo Dioulasso and examined using the KK technique, which corresponds to microscopy and the LAMP technique, to assess the sensitivity and specificity of this molecular technique. The results showed a prevalence of intestinal schistosomiasis of 8% in the study, and the Kappa coefficient obtained between the 2 techniques was 0.99, roughly equal to 1. The sensitivity and specificity of the LAMP molecular test was 100%.
Abstract: Intestinal schistosomiasis or intestinal bilharzia, mainly caused by Schistosoma mansoni, is one of the most common parasitic diseases in the world, and a neglected tropical disease (NTD). It ranks first among water-borne diseases and is the 2nd most endemic parasitic disease after malaria and according to the World Health Organization (WHO), schis...
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Research Article
Conditional Optimization of Single Cell Protein Production from Crude Oil by Pseudomonas aeruginosa
Mustafa Mohammad Haider*
,
Ashraf Anwar Rages,
Huda Al-Mergany
Issue:
Volume 9, Issue 2, June 2024
Pages:
37-47
Received:
2 May 2024
Accepted:
30 May 2024
Published:
15 August 2024
Abstract: The study was carried out to investigate the possible biodegradation of crude oil as a carbon by the bacterium Pseudomonas aeruginosa isolated from marine environment (Ras El-Menkar- Benghazi- Libya) using basal yeast extract protease peptone-3 (BYP) enriched medium. The isolated bacterium was identified and characterized according to its cultural condition and microbial biochemical properties. Different experiments were developed throughout this study to stimulate bacterial growth and production of single cell protein (SCP). The results show that the optimal concentration of crude oil as a carbon source for the highest bacterial growth (1.14g/l), and production of SCP (0.65g/l; 57.02% of the biomass dry weight) was 1%. This was required to utilize up to 50.6% of oil as a carbon source. As to the nitrogen source, the optimal concentration of ammonium chloride was 0.1%, in which the bacterial growth and SCP production increased to 1.23 g/l and 0.67 g/l respectively. The stimulating effects of organic and inorganic factors on the bacterial growth and SCP production was also tested. Addition of inorganic nutrients such as potassium phosphate (0.05%), magnesium sulphate (0.01%), and organic nutrient in the form of yeast extract (0.1%) to the fermentation medium slightly promoted the bacterial growth which reflected positively on SCP production and the percentage of the consumed crude oil, (>57%) at final pH value of 8.0. The obtained results indicated that the isolated Pseudomonas aeruginosa posses the ability to utilize the crude oil and use it as a carbon for bacterial growth and production of SCP.
Abstract: The study was carried out to investigate the possible biodegradation of crude oil as a carbon by the bacterium Pseudomonas aeruginosa isolated from marine environment (Ras El-Menkar- Benghazi- Libya) using basal yeast extract protease peptone-3 (BYP) enriched medium. The isolated bacterium was identified and characterized according to its cultural ...
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