Volume 5, Issue 1, March 2020, Page: 1-5
Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene
Mona Abdelrahman Mohamed Khaier, Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
Hanan Ahmed Daffala, Animal Resources Research Corporation, Central Veterinary Research Laboratory, Wad Medani Veterinary Research Laboratory, Wad Medani, Sudan
Abuelgasim Ibrahim Abdelhalim, Zoology Department, Faculty of Science, University of Khartoum, Khartoum, Sudan
Sumaia Mohamed Ahmed Abukashawa, Zoology Department, Faculty of Science, University of Khartoum, Khartoum, Sudan
Received: Oct. 16, 2019;       Accepted: Nov. 23, 2019;       Published: Mar. 17, 2020
DOI: 10.11648/j.bmb.20200501.11      View  380      Downloads  95
Abstract
Coccidiosis in broiler chickens highly affects the economy for both producers and consumers. The later get a low quality meat due to anaemia induced by the parasite. The disease is characterized by lesions caused by seven host specific members of the family Eimeriidae of the phylum Apicomplexa. Collected positive samples of Eimeria species from broiler chickens were identified by measuring the dimensions of the sporulated oocyst. Seven species of Eimeria were detected using this method. When DNA was extracted and species specific primers were used to amplify ITS1 gene using single specific primer PCR and multiplex PCR, only six species were identified. The accuracy of identification of broiler chicken Eimeria species using PCR is more reliable than the conventional methods like öocyst measurements or histopathology of the affected intestinal regions. This is indicated by the finding that E. brunetti which was identified morphologically by öocyst measurement, could not be identified molecularly. The mitochondrial genome sequences (ITS1) are highly suited for molecular diagnostics of coccidia and may be a potential genetic marker for molecular epidemiology of broiler chicken coccidiosis in the future in Sudan. The aim of this study is to determine and identify the species causing poultry coccidiosis in broiler chicken by traditional methods and molecular characterization using ITS.
Keywords
Apicomplexa, Broiler Chicken, Eimeria, ITS1, Poultry Coccidiosis
To cite this article
Mona Abdelrahman Mohamed Khaier, Hanan Ahmed Daffala, Abuelgasim Ibrahim Abdelhalim, Sumaia Mohamed Ahmed Abukashawa, Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene, Biochemistry and Molecular Biology. Vol. 5, No. 1, 2020, pp. 1-5. doi: 10.11648/j.bmb.20200501.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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