Volume 4, Issue 4, July 2019, Page: 59-66
Expression of Interleukins (ILs) mRNA in Otitis Media
Sung Ho Kang, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Dae Joon Lim, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Bo Hyung Kim, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Myung Sang Yu, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Shinseok Kang, Joongbu branch, Chungbuk Veterinary Service Laboratory, Chungju, Republic of Korea
Received: Oct. 3, 2019;       Accepted: Oct. 21, 2019;       Published: Oct. 25, 2019
DOI: 10.11648/j.bmb.20190404.12      View  30      Downloads  14
The purpose of this study was to investigate the expression of interleukins (ILs) cytokine in postauricular skin, mastoid mucosa, inflamed mastoid mucosa and middle ear mucosa from Otitis patients. Specimens were obtained from patients with otitis media who visited the Department of Otorhinolaryngology, Chungju Hospital, Kunkuk University School of Medicine, Chungju, Republic of Korea between August 2015 and July 2017. We examined human 38 kinds of human interleukins in this experiment. The total RNA was extracted from specimens using RNAiso Plus (Takara, Japan) according to the manufacturer's instructions. 38 ILs primers were used. IL-4, IL-8, IL-9, IL-21, IL-24 were not expressed at postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were strongly expressed in mastoid mucosa and middle ear mucosa. The degree of IL-7 gene expression may be an indicator gene in otitis media. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were involved in the inflammatory and immune processes of otitis media.
Otitis Media, Interleukins (ILs), Inflamed Mastoid Mucosa, Middle Ear Mucosa
To cite this article
Sung Ho Kang, Dae Joon Lim, Bo Hyung Kim, Myung Sang Yu, Shinseok Kang, Expression of Interleukins (ILs) mRNA in Otitis Media, Biochemistry and Molecular Biology. Vol. 4, No. 4, 2019, pp. 59-66. doi: 10.11648/j.bmb.20190404.12
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This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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