Volume 4, Issue 4, July 2019, Page: 59-66
Expression of Interleukins (ILs) mRNA in Otitis Media
Sung Ho Kang, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Dae Joon Lim, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Bo Hyung Kim, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Myung Sang Yu, Department of Otorhinolaryngology of Medicine School, Kunkuk University, Chungju, Republic of Korea
Shinseok Kang, Joongbu branch, Chungbuk Veterinary Service Laboratory, Chungju, Republic of Korea
Received: Oct. 3, 2019;       Accepted: Oct. 21, 2019;       Published: Oct. 25, 2019
DOI: 10.11648/j.bmb.20190404.12      View  30      Downloads  14
Abstract
The purpose of this study was to investigate the expression of interleukins (ILs) cytokine in postauricular skin, mastoid mucosa, inflamed mastoid mucosa and middle ear mucosa from Otitis patients. Specimens were obtained from patients with otitis media who visited the Department of Otorhinolaryngology, Chungju Hospital, Kunkuk University School of Medicine, Chungju, Republic of Korea between August 2015 and July 2017. We examined human 38 kinds of human interleukins in this experiment. The total RNA was extracted from specimens using RNAiso Plus (Takara, Japan) according to the manufacturer's instructions. 38 ILs primers were used. IL-4, IL-8, IL-9, IL-21, IL-24 were not expressed at postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were strongly expressed in mastoid mucosa and middle ear mucosa. The degree of IL-7 gene expression may be an indicator gene in otitis media. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were involved in the inflammatory and immune processes of otitis media.
Keywords
Otitis Media, Interleukins (ILs), Inflamed Mastoid Mucosa, Middle Ear Mucosa
To cite this article
Sung Ho Kang, Dae Joon Lim, Bo Hyung Kim, Myung Sang Yu, Shinseok Kang, Expression of Interleukins (ILs) mRNA in Otitis Media, Biochemistry and Molecular Biology. Vol. 4, No. 4, 2019, pp. 59-66. doi: 10.11648/j.bmb.20190404.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Minovi A, Dazert S. Diseases of the middle ear in childhood Review. GMS Current Topics in Otorhinolaryngology - Head and Neck Surgery. 2014; 13: 1-29.
[2]
Coker TR, Chan LS, Newberry SJ, et al. Diagnosis, microbial epidemiology, and antibiotic treatment of acute otitis media in children: a systematic review. JAMA. 2010; 304 (19): 2161-2169.
[3]
Casey JR, Pichichero ME. Changes in frequency and pathogens causing acute otitis media in 1995-2003. Pediatr Infect Dis J. 2004; 23 (9): 824-828.
[4]
Commins SP, Borish L, Steinke JW. Immunologic messenger molecules: Cytokines, interferons, and chemokines. J Allergy Clin. Immunol. 2010; 125: 53–72.
[5]
Akdis M, Aab A, Altunbulakli C, Azkur K, Costa RA. Crameri R, Duan S, Eiwegger T, Eljaszewicz A, Ferstl R, Frei R, Garbani M, Globinska A, Hess L, Huitema C, Kubo T, Komlosi Z, Konieczna P, Kovacs N, Kucuksezer UC, Meyer N, Morita H, Olzhausen J, Mahony LO, Pezer M, Prati M, Rebane A, Rhyner C, Rinaldi A, Sokolowska Milena, Stanic B, Sugita K, Treis A, Veen W, Wanke K, Wawrzyniak M, Wawrzyniak P, Wirz OF. Zakzuk JS, Akdis CA. Interleukins (from IL-1 to IL-38), interferons, transforming growth factor b, and TNF-a: Receptors, functions, and roles in diseases. Fundamentals of allergy and immunology. 2016; 984-1011.
[6]
Dinarello CA. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood. 2011; 117 (14): 3720-3732.
[7]
Veerdonka FL, Stoeckmanc AK, Wu G, Boeckermann AN, Azama T, Netea MG, Joosten LA, Meer WM, Hao R, Kalabokis V, Dinarelloa CA. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist. PNAS, 2012; 109 (8): 3001–3005.
[8]
Ouyang W, Rutz S, Crellin NK, Valdez PA, Hymowitz SG. Regulation and functions of the IL-10 family of cytokines in inflammation and disease. Annu Rev Immunol 2011; 29: 71-109.
[9]
Vignali DA, Kuchroo VK. IL-12 family cytokines: immunological playmakers. Nat Immunol. 2012; 13 (8): 722-728.
[10]
Thomas AW. Type 2 cytokines: mechanisms and therapeutic strategies. Nature Reviews Immunology. 2015; 15: 271–282.
[11]
Weaver CT, Hatton RD, Mangan P, Harrington LE. IL-17 Family Cytokines and the expanding diversity of effector T Cell lineages. Annu. Rev. Immunol. 2007; 25: 821–852.
[12]
Moseley TA, Haudenschild DR, Rose L, Reddi AH. Interleukin-17 family and IL-17 receptors. Cytokine & growth factor reviews, 2003; 14 (2): 155-174.
[13]
Holmquist J, Jarlstedt J, Tjellström A. Surgery of the mastoid in ears with middle ear effusion. Ann Otol Rhinol Laryngol Suppl. 1980; 89 (3): 322-323.
[14]
Fooken Jensen PV, Gaihede M. Congestion of mastoid mucosa and influence on middle ear pressure - Effect of retroauricular injection of adrenaline. Hear Res. 2016; 340: 121-126.
[15]
Lim DJ. Functional morphology of the mucosa of the middle ear and Eustachian tube. Ann Otol Rhinol Laryngol. 1976; 85 (2): 36-43.
[16]
Zhang JM, AnJianxiong. Cytokines, Inflammation and Pain. Int Anesthesiol Clin. 2007; 45 (2): 27–37.
[17]
Monin L, Gaffen S L, IL-17 family cytokines: signaling mechanisms, biological activities and therapeutic implications. Cold Spring Harb Perspect Biol. 2018; 10 (4).
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