Volume 3, Issue 5, September 2018, Page: 63-70
Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers
El-Kawokgy Tahany Mohamed Abou El-Fath, Microbial Genetics Department, National Research Centre, Giza, Egypt
Ibrahim Shafik Darwish, Molecular Genetics and Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt
Ashraf Gamil Attallah, Microbial Genetics Department, National Research Centre, Giza, Egypt
Received: Nov. 10, 2018;       Accepted: Dec. 7, 2018;       Published: Jan. 10, 2019
DOI: 10.11648/j.bmb.20180305.11      View  85      Downloads  23
Abstract
Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.
Keywords
Bacillus Thuringiensis, Bacillus Sphaericus, Genetic Diversity, ISSR and SCoT Marker
To cite this article
El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah, Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers, Biochemistry and Molecular Biology. Vol. 3, No. 5, 2018, pp. 63-70. doi: 10.11648/j.bmb.20180305.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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