Volume 3, Issue 3, May 2018, Page: 49-55
The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis
Larisa Mikhailovna Somova, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Irina Nikolaevna Lyapun, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Elena Igorevna Drobot, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Felix Nikolayevich Shubin, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Natalya Gennadyevna Plekhova, Ministry of Health of Russia, Pacific Medical University, Vladivostok, Russian Federation
Received: Sep. 18, 2018;       Accepted: Oct. 5, 2018;       Published: Nov. 1, 2018
DOI: 10.11648/j.bmb.20180303.11      View  193      Downloads  17
Abstract
In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.
Keywords
Metabolic Activity, Innate Immunity Cells, ATPase, Lactate Dehydrogenase, Myelo Peroxidase, Nitric Oxide Metabolites, Stimulation Index (Т), Variability, Plasmid Types of Yersinia Pseudotu Berculosis
To cite this article
Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova, The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis, Biochemistry and Molecular Biology. Vol. 3, No. 3, 2018, pp. 49-55. doi: 10.11648/j.bmb.20180303.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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