Volume 2, Issue 6, November 2017, Page: 94-101
Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis
Feibing Wang, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Bin Weng, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Qi Wang, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Yuxiu Ye, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Jing Zhang, School of Chemical Engineering, Huaiyin Institute of Technology, Huai’an, China
Gaolei Ren, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Bowen Wang, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Qing Zhou, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Xinhong Chen, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Received: Sep. 5, 2017;       Accepted: Oct. 18, 2017;       Published: Nov. 22, 2017
DOI: 10.11648/j.bmb.20170206.15      View  1366      Downloads  83
Abstract
A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.
Keywords
Arabidopsis, Constitutive Expression, Grapevine, Starch Content, VvAATP
To cite this article
Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, Gaolei Ren, Bowen Wang, Qing Zhou, Xinhong Chen, Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis, Biochemistry and Molecular Biology. Vol. 2, No. 6, 2017, pp. 94-101. doi: 10.11648/j.bmb.20170206.15
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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