Volume 1, Issue 3, November 2016, Page: 34-38
Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor
Fang-Di Cong, Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Jie Kang, Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Wu-Dan Bi, Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Tao Li, Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Ping Li, Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Received: Oct. 30, 2016;       Accepted: Nov. 15, 2016;       Published: Dec. 14, 2016
DOI: 10.11648/j.bmb.20160103.12      View  2365      Downloads  65
Abstract
A bioreactor with Pseudomonas cepacia lipase (PCL) immobilized on the inner wall was conveniently prepared by adding lipase powder and the right amount of water to a conical flask and keeping it with mouth open in an incubator shaker at 37°C and 170 rpm for more than 10 h. The bioreactor was employed on resolution of (R, S)-1-chloro-3-(1-naphthyloxy)-2-propanol by catalyzing transesterification of it with vinyl acetate. It was showed that the wall-PCL behaved an excellent catalytic activity being 10 folds of native PCL in terms of conversion, and high enantioselectivity E = 110. And also the depressed activity of immobilized PCL owing to frequently use in organic phase could be reactivated easily by again shaking bioreactor under the aforesaid conditions after adding water. The enhanced activity was attributed to the simulation on interfacial activation mechanism of lipases at water/oil interface.
Keywords
Lipase, Activity, Enantioselectivity, Immobilization, Pseudomonas Cepacia Lipase, Bioreactor
To cite this article
Fang-Di Cong, Jie Kang, Wu-Dan Bi, Tao Li, Ping Li, Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor, Biochemistry and Molecular Biology. Vol. 1, No. 3, 2016, pp. 34-38. doi: 10.11648/j.bmb.20160103.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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