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Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique

Received: 29 July 2021     Accepted: 11 September 2021     Published: 23 September 2021
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Abstract

Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.

Published in International Journal of Biomedical Science and Engineering (Volume 9, Issue 3)
DOI 10.11648/j.ijbse.20210903.14
Page(s) 73-77
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Atherosclerosis, Rabbits, Balloon Endothelial Injury, Animal Model, CT Imaging

References
[1] World Health Organization, 2009. Cardiovascular Diseases [J]. Accessed June 17, 2010.
[2] Burke AP, Farb A, Malcom GT, et al: Plaque rupture and sudden death related to exertion in men withcoronary artery disease. JAMA 1999; 281: 921-926.
[3] D. Mozaffarian, E. J. Benjamin, A. S. Go, D. K. Arnett, M. J. Blaha, M. Cushman, S. R. Das, S. de Ferranti, J. -P. Després, H. J. Fullerton, V. J. Howard, M. D. Huffman, C. R. Isasi, M. C. Jiménez, S. E. Judd, B. M. Kissela, J. H. Lichtman, L. D. Lisabeth, S. Liu, R. H. Mackey, D. J. Magid, D. K. McGuire, E. R. Mohler, C. S. Moy, P. Muntner, M. E. Mussolino, K. Nasir, R. W. Neumar, G. Nichol, L. Palaniappan, D. K. Pandey, M. J. Reeves, C. J. Rodriguez, W. Rosamond, P. D. Sorlie, J. Stein, A. Towfighi, T. N. Turan, S. S. Virani, D. Woo, R. W. Yeh, M. B. Turner, Heart disease and stroke statistics-2016 update, Circulation (2015).
[4] Cui Wenyu, Du ran. Study on the animal model of carotid atherosclerosis Progress [J]. China urban and rural enterprise health, 2016 (6): 31-32.
[5] Leber AW, von Ziegler F, Becker A et al (2008) Characteristics of coronary plaques before angiographic progression determined by multi-slice CT. Int J Cardiovasc Imaging 24: 423-428.
[6] P. Libby, Inflammation in atherosclerosis, Nature 420 (6917) (2002) 868-874.
[7] A. G. Horti, Y. Gao, H. Kuwabara, Y. Wang, S. Abazyan, R. P. Yasuda, T. Tran, Y. Xiao, N. Ahibzada, D. P. Holt, K. J. Kellar, M. V. Pletnikov, M. G. Pomper, D. F. Wong and R. F. Dannals, 18F-ASEM, a radiolabeled antagonist for imaging the α7-nicotinic acetylcholine receptor with PET, JNucl. Med. 55 (4) (2014) 672-677.
[8] Chen Hua, Xie Zhongchen, Huang Guangyong, et al. Atherosclerosis in Wuzhishan miniature pig Establishment of metamodel. Experimental animal science, 2007, 24 (6): 39-43.
[9] Bergen W G, Mersanmann H J. Comparative aspect of lipid metabolism: import on contemporary research and use of animal models [J]. Journal of Nutrition. 2005, 135 (11): 2499-2502.
[10] Forster BA, Weinberg PD. Changes with age in the influence of endogenous nitricoxide on transport ProPerties of therabbit aortic wall near branches. Arterioscler Thromb Vasc Biol. 1997; 17: 1361-1368.
[11] Haunstetter A, Izumo S. Apoptosis: Basic mechanisms and implicatio ns for cardiovasculardisease. Circ Res. 1998; 82: 1111-1129.
[12] FAN J, SHIMOYAMADA H, SUN H, et al. Transgenic rabbits expressing human apolipoprotein (a) develop more extensive atherosclerotic lesions in response to a cholesterol-rich diet. Arterioscler Thromb Vascular Biol, 2001, 21: 88-94.
[13] Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature. 1993; 362 (6423): 801-809.
[14] Schwartz CJ, Valente AJ, Sprague EA. A modern view of atherosclerosis. Am J Card. 1993; 71 (6): B9-B14.
Cite This Article
  • APA Style

    Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, et al. (2021). Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. International Journal of Biomedical Science and Engineering, 9(3), 73-77. https://doi.org/10.11648/j.ijbse.20210903.14

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    ACS Style

    Dawei Wang; Tao Yang; Xiangyi Chen; Feng Guo; Liujun Jia, et al. Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. Int. J. Biomed. Sci. Eng. 2021, 9(3), 73-77. doi: 10.11648/j.ijbse.20210903.14

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    AMA Style

    Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, et al. Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. Int J Biomed Sci Eng. 2021;9(3):73-77. doi: 10.11648/j.ijbse.20210903.14

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  • @article{10.11648/j.ijbse.20210903.14,
      author = {Dawei Wang and Tao Yang and Xiangyi Chen and Feng Guo and Liujun Jia and Guangxin Yue and Ying Kui Liang and Xin Wang},
      title = {Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique},
      journal = {International Journal of Biomedical Science and Engineering},
      volume = {9},
      number = {3},
      pages = {73-77},
      doi = {10.11648/j.ijbse.20210903.14},
      url = {https://doi.org/10.11648/j.ijbse.20210903.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20210903.14},
      abstract = {Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique
    AU  - Dawei Wang
    AU  - Tao Yang
    AU  - Xiangyi Chen
    AU  - Feng Guo
    AU  - Liujun Jia
    AU  - Guangxin Yue
    AU  - Ying Kui Liang
    AU  - Xin Wang
    Y1  - 2021/09/23
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijbse.20210903.14
    DO  - 10.11648/j.ijbse.20210903.14
    T2  - International Journal of Biomedical Science and Engineering
    JF  - International Journal of Biomedical Science and Engineering
    JO  - International Journal of Biomedical Science and Engineering
    SP  - 73
    EP  - 77
    PB  - Science Publishing Group
    SN  - 2376-7235
    UR  - https://doi.org/10.11648/j.ijbse.20210903.14
    AB  - Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.
    VL  - 9
    IS  - 3
    ER  - 

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Author Information
  • Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, People’s Republic of China

  • Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China

  • Department of Nuclear Medicine, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang Central People’s Hospital, Yichang, People’s Republic of China

  • Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, People’s Republic of China

  • Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China

  • Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China

  • Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, People’s Republic of China

  • Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China

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