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Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells

Received: 28 November 2023     Accepted: 23 December 2023     Published: 8 January 2024
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Abstract

Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.

Published in Biochemistry and Molecular Biology (Volume 9, Issue 1)
DOI 10.11648/j.bmb.20240901.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lipopolysaccharide, Signaling Activities, cJun N-terminal Kinase, Gene Expression

References
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Cite This Article
  • APA Style

    Zindel, K., Whiteman, S., Wiewel, K., Turner, A., McGivern, M., et al. (2024). Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochemistry and Molecular Biology, 9(1), 1-6. https://doi.org/10.11648/j.bmb.20240901.11

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    ACS Style

    Zindel, K.; Whiteman, S.; Wiewel, K.; Turner, A.; McGivern, M., et al. Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochem. Mol. Biol. 2024, 9(1), 1-6. doi: 10.11648/j.bmb.20240901.11

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    AMA Style

    Zindel K, Whiteman S, Wiewel K, Turner A, McGivern M, et al. Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochem Mol Biol. 2024;9(1):1-6. doi: 10.11648/j.bmb.20240901.11

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  • @article{10.11648/j.bmb.20240901.11,
      author = {Kristin Zindel and Sarah Whiteman and Kurstin Wiewel and Anne Turner and Meghan McGivern and Suzanne Stapleton and Marti Jett and Chanaka Mendis},
      title = {Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells},
      journal = {Biochemistry and Molecular Biology},
      volume = {9},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.bmb.20240901.11},
      url = {https://doi.org/10.11648/j.bmb.20240901.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20240901.11},
      abstract = {Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.
    },
     year = {2024}
    }
    

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    T1  - Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells
    AU  - Kristin Zindel
    AU  - Sarah Whiteman
    AU  - Kurstin Wiewel
    AU  - Anne Turner
    AU  - Meghan McGivern
    AU  - Suzanne Stapleton
    AU  - Marti Jett
    AU  - Chanaka Mendis
    Y1  - 2024/01/08
    PY  - 2024
    N1  - https://doi.org/10.11648/j.bmb.20240901.11
    DO  - 10.11648/j.bmb.20240901.11
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
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    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20240901.11
    AB  - Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.
    
    VL  - 9
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry, UW-Platteville, Platteville, United States of America

  • Department of Chemistry, UW-Platteville, Platteville, United States of America

  • Department of Chemistry, UW-Platteville, Platteville, United States of America

  • Department of Chemistry, UW-Platteville, Platteville, United States of America

  • Department of Chemistry, UW-Platteville, Platteville, United States of America

  • Department of Chemistry, UW-Platteville, Platteville, United States of America

  • Department of Molecular Pathology, Walter Reed Army Institute of Research, Silver Spring, United State of America

  • Department of Chemistry, UW-Platteville, Platteville, United States of America

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